Ultrastructural observations of enzymatically treated human blastocysts: zona-free blastocyst transfer and rescue of blastocysts with hatching difficulties
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چکیده
منابع مشابه
Ultrastructural observations of enzymatically treated human blastocysts: zona-free blastocyst transfer and rescue of blastocysts with hatching difficulties.
Enzymatic treatment of the zona pellucida to either soften or remove totally the zona before blastocyst transfer has resulted in high implantation rates. The zona is usually completely dissolved after 1.5 min exposure with 10 IU pronase at 37 degrees C. Since there may be concerns that pronase treatment for periods of 1.5 min or longer may cause adverse effects on the trophectoderm (TE) and inn...
متن کاملOngoing normal pregnancy after transfer of zona-free blastocysts: implications for embryo transfer in the human.
Recent reports suggest that transfer of day 5 blastocysts improves implantation rates in in-vitro fertilization programmes. This paper reports a successful ongoing pregnancy after the transfer of zona-free day 6 expanded and hatching blastocysts. The patient was 37 years old and had undergone six stimulated and two thaw cycles previously, all of which had failed. Stimulation was by down-regulat...
متن کاملHigh implantation and pregnancy rates with transfer of human hatching day 6 blastocysts.
Although improved outcomes of IVF after transfer of blastocysts has been reported (1, 2), it remains uncertain which blastocyst can develop into a viable fetus. At present, expansion of the blastocyst is the most practical selection criterion. However, if extended culture of embryos produces many expanded-stage blastocysts, it is not easy to decide which blastocysts to transfer (Fig. 1A). In ad...
متن کاملHatching and Distribution of Actin Filaments in Mouse Blastocysts Treated with Cytochalasin B
It is generally accepted that the hatching of mouse blastocysts is accompanied by regional dissolution of the zona pellucida by a trypsin-like proteinase synthesized in trophectoderm cells (Perona and Wassarman, 1986; Hogan et al., 1994), and trophectoderm cells protrude from the dissolved hole of the zona pellucida (Orsini and McLaren, 1967; McLaren, 1970; Niimura and Fujii, 1997). Then a slit...
متن کاملHatching status before embryo transfer is not correlated with implantation rate in chromosomally screened blastocysts.
STUDY QUESTION Do the reproductive outcomes from the transfer of fully hatched (FH) blastocysts differ from those of not fully hatched (NFH) blastocysts? SUMMARY ANSWER Biochemical pregnancy rate (BPR), implantation rate (IR), live birth rate (LBR) and early pregnancy loss (EPL) rate are similar in FH and NFH single euploid blastocyst embryo transfers. WHAT IS KNOWN ALREADY The use of exten...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2001
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/16.3.540